Title: Validating Cellular Models Using Live Cell Metabolic Analysis
Tuesday, December 4, 2018
Harvey Morse Auditorium
Presenter: Ned Jastromb, Agilent Technologies – Product Manager, New Platforms, Cell Analysis Division
Recent evidence in the scientific literature illustrates the role that cellular metabolism plays in controlling cell fate and function. Relevant disease models require complete characterization and validation of cellular function and performance. Improving reprogramming efficiencies, measuring the state of a differentiated cell population, and determining cellular maturity along the differentiation axis can be measured by distinguishing the contribution of glycolysis or mitochondrial respiration which collectively support the cell’s energy production needs. These needs shift as cells transition from a stem cell to a progenitor to a differentiated cell type.
Agilent Seahorse XF Extracellular Flux Analyzers measure cell metabolism, simultaneously measuring the two major energy-producing pathways within the cell - mitochondrial respiration and glycolysis - in live cells, in real time.
The acceptance of Seahorse XF Analyzers as the industry standard for measuring cell metabolism is evident by the increasing rate of scientific publications that contain references to XF data. Using XF technology researchers can now more easily study cell metabolism and mitochondrial dysfunction and gain greater understanding about disease progression, impacting research in many disease areas, such as cancer, immunology, diabetes, Alzheimer's, Parkinson's, Huntington's, and cardiovascular disease.
In this XF technology workshop you will learn:
- How XF technology measures both respiration and glycolysis in live cells
- How to conduct simple and powerful XF stress tests
- How XF data is being used to further stem cell research with real world examples