Friday, September 28, 2018
Deciphering the biology that drives response to immunotherapy using multiplexed immunofluorescence – Phenoptics update
Linden Wyatt, PhD, Field Applications Scientist, Quantitative Pathology Solutions, PerkinElmer
The tumor microenvironment remains a complex and challenging entity supporting tumor growth, immune escape, and even metastatic potential. Recently, the emergence of novel immunotherapies such as ipilimumab and nivolumab demonstrating benefit in some of the most aggressive phases of cancer progression, have demonstrated that approaches directing the patient’s own immune system against tumors are proving valuable. However, further advances will require a detailed understanding of the cell-to-cell interactions occurring in the tumor microenvironment, including the characterization of the location and status of immune cells relative to tumor. To achieve this, a practical and simplified method for high-throughput quantitative immunofluorescence of up to 6 biomarkers in a single tissue section using standard unlabeled primary antibodies will be described. Advances toward an automated, validated and standardized approach will be presented, suggesting that the technology is well-suited to reliable quantitative assessments of FFPE, to support small to large studies in translational research.